› 5utt

X-ray diffraction
1.7Å resolution

SrtA sortase from Actinomyces oris

Released:
DOI: 10.2210/pdb5utt/pdb
PDB entry 5utt coloured by chain, front view.
PDB entry 5utt coloured by chain, side view.
PDB entry 5utt coloured by chain, top view.
Source organism: Actinomyces oris
Primary publication:
Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes.
Chang C, Wu C, Osipiuk J, Siegel SD, Zhu S, Liu X, Joachimiak A, Clubb RT, Das A, Ton-That H
Proc Natl Acad Sci U S A 116 18041-18049 (2019)
PMID: 31427528

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Class E sortase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 20.93 KDa
Source organism: Actinomyces oris
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0X8K1J2 (Residues: 71-257; Coverage: 73%)
Gene name: RMW62_06580
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

1 bound ligand:
Ligand CL 12 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 62.183Å b: 75.085Å c: 77.652Å
α: 90° β: 89.96° γ: 90°
R-values:
R R work R free
0.162 0.16 0.205
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

3 review citations

New Paradigms of Pilus Assembly Mechanisms in Gram-Positive Actinobacteria.
Ramirez et al. (2020)
2 more

4 mentions without citation

SARS-CoV-2 3D database: understanding the coronavirus proteome and evaluating possible drug targets.
Alsulami et al. (2021)
3 more