› 5uvm

X-ray diffraction
2.3Å resolution

HIT family hydrolase from Clostridium thermocellum Cth-393

Released:
DOI: 10.2210/pdb5uvm/pdb
PDB entry 5uvm coloured by chain, front view.
PDB entry 5uvm coloured by chain, side view.
PDB entry 5uvm coloured by chain, top view.
Source organism: Acetivibrio thermocellus DSM 1313
Entry authors: Habel J, Tempel W, Liu Z-J, Rose J, Wang B-C, Southeast Collaboratory for Structural Genomics (SECSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HIT domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.39 KDa
Source organism: Acetivibrio thermocellus DSM 1313
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DF72 (Residues: 2-114; Coverage: 99%)
Gene name: Cthe_1369
Sequence domains: Scavenger mRNA decapping enzyme C-term binding
Structure domains: HIT-like

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand UNX 31 x UNX
Ligand ADN 1 x ADN
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P4122
Unit cell:
a: 78.804Å b: 78.804Å c: 114.885Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.233
Expression system: Escherichia coli

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