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› 5uxm

X-ray diffraction
1.54Å resolution

Type II DAH7PS from Pseudomonas aeruginosa with Trp bound

Released:
DOI: 10.2210/pdb5uxm/pdb
Model geometry
Fit model/data
PDB entry 5uxm coloured by chain, front view.
PDB entry 5uxm coloured by chain, side view.
PDB entry 5uxm coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
A Pseudoisostructural Type II DAH7PS Enzyme from Pseudomonas aeruginosa: Alternative Evolutionary Strategies to Control Shikimate Pathway Flux.
Sterritt OW, Kessans SA, Jameson GB, Parker EJ
Biochemistry 57 2667-2678 (2018)
PMID: 29608284

Function and Biology Details

Reaction catalysed: 
D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase Chain: A
Molecule details ›
Chain: A
Length: 453 amino acids
Theoretical weight: 50.2 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I000 (Residues: 2-448; Coverage: 100%)
Gene name: PA2843
Sequence domains: Class-II DAHP synthetase family

Ligands and Environments

5 bound ligands:
Ligand PEP 1 x PEP
Ligand TRP 1 x TRP
Ligand PO4 2 x PO4
Ligand CO 1 x CO
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: I222
Unit cell:
a: 81.68Å b: 100.37Å c: 115.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Mycobacterium tuberculosis Shikimate Pathway Enzymes as Targets for the Rational Design of Anti-Tuberculosis Drugs.
Nunes et al. (2020)
4 other articles

2 mentions without citation

Structural and functional characterisation of the entry point to pyocyanin biosynthesis in Pseudomonas aeruginosa defines a new 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase subclass.
Sterritt et al. (2018)
1 more