› 5v47

X-ray diffraction
1.84Å resolution

Crystal structure of the SR1 domain of lizard sacsin

Released:
DOI: 10.2210/pdb5v47/pdb
PDB entry 5v47 coloured by chain, front view.
PDB entry 5v47 coloured by chain, side view.
PDB entry 5v47 coloured by chain, top view.
Source organism: Anolis carolinensis
Primary publication:
Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations.
Ménade M, Kozlov G, Trempe JF, Pande H, Shenker S, Wickremasinghe S, Li X, Hojjat H, Dicaire MJ, Brais B, McPherson PS, Wong MJH, Young JC, Gehring K
J Biol Chem 293 12832-12842 (2018)
PMID: 29945973

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sacsin molecular chaperone Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 28.75 KDa
Source organism: Anolis carolinensis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: G1KBF9 (Residues: 96-343; Coverage: 5%)
Gene name: SACS

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 160.779Å b: 38.164Å c: 114.721Å
α: 90° β: 131.55° γ: 90°
R-values:
R R work R free
0.197 0.195 0.234
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

The Classification of Autosomal Recessive Cerebellar Ataxias: a Consensus Statement from the Society for Research on the Cerebellum and Ataxias Task Force.
Beaudin et al. (2019)
2 more

1 mention without citation

Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations.
Ménade et al. (2018)