› 5v4b

X-ray diffraction
2.6Å resolution

Crystal structure of the Skp1-FBXW7-DISC1 complex

Released:
DOI: 10.2210/pdb5v4b/pdb
PDB entry 5v4b coloured by chain, front view.
PDB entry 5v4b coloured by chain, side view.
PDB entry 5v4b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
FBXW7 regulates DISC1 stability via the ubiquitin-proteosome system.
Yalla K, Elliott C, Day JP, Findlay J, Barratt S, Hughes ZA, Wilson L, Whiteley E, Popiolek M, Li Y, Dunlop J, Killick R, Adams DR, Brandon NJ, Houslay MD, Hao B, Baillie GS
Mol Psychiatry 23 1278-1286 (2018)
PMID: 28727686

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dodecamer
PDBe Complex ID:
PDB-CPX-159045 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63208 (Residues: 1-69, 82-163; Coverage: 89%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
F-box/WD repeat-containing protein 7 Chain: B
Molecule details ›
Chain: B
Length: 444 amino acids
Theoretical weight: 50.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q969H0 (Residues: 263-706; Coverage: 63%)
Gene names: FBW7, FBX30, FBXW7, SEL10
Sequence domains:
Structure domains:
Disrupted in schizophrenia 1 protein Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NRI5 (Residues: 193-207; Coverage: 2%)
Gene names: DISC1, KIAA0457

Ligands and Environments

2 bound ligands:
Ligand SO4 9 x SO4
Ligand IMD 1 x IMD
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4122
Unit cell:
a: 233.514Å b: 233.514Å c: 108.438Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.219
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

E3 Ligase Ligands for PROTACs: How They Were Found and How to Discover New Ones.
Ishida et al. (2021)
3 more

3 mentions without citation

FBXW7 regulates DISC1 stability via the ubiquitin-proteosome system.
Yalla et al. (2018)
2 more