› 5vaq

X-ray diffraction
2.61Å resolution

Crystal Structure of Beta-Klotho in Complex with FGF21CT

Released:
DOI: 10.2210/pdb5vaq/pdb
PDB entry 5vaq coloured by chain, front view.
PDB entry 5vaq coloured by chain, side view.
PDB entry 5vaq coloured by chain, top view.
Source organisms:
Primary publication:
Structures of β-klotho reveal a 'zip code'-like mechanism for endocrine FGF signalling.
Lee S, Choi J, Mohanty J, Sousa LP, Tome F, Pardon E, Steyaert J, Lemmon MA, Lax I, Schlessinger J
Nature 553 501-505 (2018)
PMID: 29342135

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-212552 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-klotho Chain: A
Molecule details ›
Chain: A
Length: 954 amino acids
Theoretical weight: 109.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q86Z14 (Residues: 30-983; Coverage: 91%)
Gene name: KLB
Sequence domains: Glycosyl hydrolase family 1
Nb914 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 14.84 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Fibroblast growth factor 21 Chain: C
Molecule details ›
Chain: C
Length: 24 amino acids
Theoretical weight: 2.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NSA1 (Residues: 186-209; Coverage: 13%)
Gene names: FGF21, UNQ3115/PRO10196

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 48.647Å b: 145.487Å c: 213.827Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.229
Expression systems:
  • Homo sapiens
  • Escherichia coli
  • Not provided

Quick links

Citations

51 review citations

The Klotho proteins in health and disease.
Kuro-O M. (2019)
50 more

7 mentions without citation

Structures of β-klotho reveal a 'zip code'-like mechanism for endocrine FGF signalling.
Lee et al. (2018)
6 more