› 5vkm

X-ray diffraction
2.2Å resolution

Crystal structure of human CD22 Ig domains 1-3 in complex with alpha 2-6 sialyllactose

Released:
DOI: 10.2210/pdb5vkm/pdb
PDB entry 5vkm coloured by chain, front view.
PDB entry 5vkm coloured by chain, side view.
PDB entry 5vkm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of human CD22 function and therapeutic targeting.
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP
Nat Commun 8 764 (2017)
PMID: 28970495

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148914 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
B-cell receptor CD22 Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P20273 (Residues: 20-330; Coverage: 38%)
Gene names: CD22, SIGLEC2
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 124.283Å b: 57.912Å c: 48.081Å
α: 90° β: 106.99° γ: 90°
R-values:
R R work R free
0.215 0.21 0.254
Expression system: Homo sapiens

Quick links

Citations

28 review citations

Glycosylation in health and disease.
Reily et al. (2019)
27 more

11 mentions without citation

Current Status on Therapeutic Molecules Targeting Siglec Receptors.
Lenza et al. (2020)
10 more