5vn3

Electron Microscopy
3.7Å resolution

Cryo-EM model of B41 SOSIP.664 in complex with soluble CD4 (D1-D2) and fragment antigen binding variable domain of 17b

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-207657 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (10 distinct):
17b Fab light chain Chains: L, N, O
Molecule details ›
Chains: L, N, O
Length: 214 amino acids
Theoretical weight: 23.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 153 amino acids
Theoretical weight: 17.36 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: B3UEZ6 (Residues: 516-668; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
Envelope glycoprotein gp160 Chains: G, I, J
Molecule details ›
Chains: G, I, J
Length: 516 amino acids
Theoretical weight: 57.7 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: B3UES2 (Residues: 29-509; Coverage: 56%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
T-cell surface glycoprotein CD4 Chains: C, E, F
Molecule details ›
Chains: C, E, F
Length: 185 amino acids
Theoretical weight: 20.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01730 (Residues: 26-208; Coverage: 42%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins
17b Fab heavy chain Chains: H, K, M
Molecule details ›
Chains: H, K, M
Length: 229 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-8713
Expression system: Homo sapiens