› 5vpr

X-ray diffraction
2.05Å resolution

Crystal Structure of Cysteine desulfurase from Elizabethkingia anophelis with covalently bound pyridoxal phosphate

Released:
DOI: 10.2210/pdb5vpr/pdb
PDB entry 5vpr coloured by chain, front view.
PDB entry 5vpr coloured by chain, side view.
PDB entry 5vpr coloured by chain, top view.
Source organism: Elizabethkingia anophelis NUHP1
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100522 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.47 KDa
Source organism: Elizabethkingia anophelis NUHP1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A077EMQ8 (Residues: 1-406; Coverage: 100%)
Gene name: BD94_3973
Sequence domains: Aminotransferase class-V

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 84.18Å b: 82.04Å c: 70.12Å
α: 90° β: 99.44° γ: 90°
R-values:
R R work R free
0.172 0.17 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links