› 5vt2

X-ray diffraction
2.3Å resolution

Crystal structure of growth differentiation factor

Released:
DOI: 10.2210/pdb5vt2/pdb
PDB entry 5vt2 coloured by chain, front view.
PDB entry 5vt2 coloured by chain, side view.
PDB entry 5vt2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Long-acting MIC-1/GDF15 molecules to treat obesity: Evidence from mice to monkeys.
Xiong Y, Walker K, Min X, Hale C, Tran T, Komorowski R, Yang J, Davda J, Nuanmanee N, Kemp D, Wang X, Liu H, Miller S, Lee KJ, Wang Z, Véniant MM
Sci Transl Med 9 (2017)
PMID: 29046435

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Growth/differentiation factor 15 Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99988 (Residues: 197-308; Coverage: 40%)
Gene names: GDF15, MIC1, PDF, PLAB, PTGFB
Sequence domains: Transforming growth factor beta like domain

Ligands and Environments

1 bound ligand:
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P3121
Unit cell:
a: 64.72Å b: 64.72Å c: 172.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.242
Expression system: Escherichia coli BL21

Quick links

Citations

28 review citations

The endocrine function of adipose tissues in health and cardiometabolic disease.
Scheja et al. (2019)
27 more