5vzl

Electron Microscopy
3.9Å resolution

cryo-EM structure of the Cas9-sgRNA-AcrIIA4 anti-CRISPR complex

Released:
Related structures: EMD-8749

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-104175 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1369 amino acids
Theoretical weight: 158.77 KDa
Source organism: Streptococcus pyogenes M1 GAS
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
phage anti-CRISPR AcrIIA4 Chain: C
Molecule details ›
Chain: C
Length: 87 amino acids
Theoretical weight: 10.18 KDa
Source organism: Bacteriophage sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2D0TCG7 (Residues: 1-87; Coverage: 100%)
single guide RNA (116-MER) Chain: B
Molecule details ›
Chain: B
Length: 118 nucleotides
Theoretical weight: 38.29 KDa

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-8749
Expression system: Escherichia coli