› 5w0r

X-ray diffraction
2.4Å resolution

Crystal structure of MBP fused activation-induced cytidine deaminase (AID) in complex with cacodylic acid

Released:
DOI: 10.2210/pdb5w0r/pdb
PDB entry 5w0r coloured by chain, front view.
PDB entry 5w0r coloured by chain, side view.
PDB entry 5w0r coloured by chain, top view.
Source organisms:
Primary publication:
AID Recognizes Structured DNA for Class Switch Recombination.
Qiao Q, Wang L, Meng FL, Hwang JK, Alt FW, Wu H
Mol Cell 67 361-373.e4 (2017)
PMID: 28757211

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein; Single-stranded DNA cytosine deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 61.66 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q9GZX7 (Residues: 13-181; Coverage: 85%)
Gene names: AICDA, AID, JW3994, b4034, malE
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CAC 2 x CAC
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 36.96Å b: 179.07Å c: 188.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.239
Expression system: Spodoptera frugiperda

Quick links

Citations

30 review citations

V(D)J recombination, somatic hypermutation and class switch recombination of immunoglobulins: mechanism and regulation.
Chi et al. (2020)
29 more

4 mentions without citation

AID Recognizes Structured DNA for Class Switch Recombination.
Qiao et al. (2017)
3 more