› 5w1l

X-ray diffraction
2.88Å resolution

Echinococcus granulosus thioredoxin glutathione reductas (egTGR) with Gold

Released:
DOI: 10.2210/pdb5w1l/pdb
PDB entry 5w1l coloured by chain, front view.
PDB entry 5w1l coloured by chain, side view.
PDB entry 5w1l coloured by chain, top view.
Source organism: Echinococcus granulosus
Primary publication:
The Enzymatic and Structural Basis for Inhibition of Echinococcus granulosus Thioredoxin Glutathione Reductase by Gold(I).
Salinas G, Gao W, Wang Y, Bonilla M, Yu L, Novikov A, Virginio VG, Ferreira HB, Vieites M, Gladyshev VN, Gambino D, Dai S
Antioxid Redox Signal 27 1491-1504 (2017)
PMID: 28463568

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183116 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
thioredoxin-disulfide reductase Chain: A
Molecule details ›
Chain: A
Length: 586 amino acids
Theoretical weight: 64.56 KDa
Source organism: Echinococcus granulosus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q869D7 (Residues: 34-617; Coverage: 94%)
Gene name: TGR
Sequence domains:
Structure domains:
thioredoxin-disulfide reductase Chain: B
Molecule details ›
Chain: B
Length: 584 amino acids
Theoretical weight: 64.34 KDa
Source organism: Echinococcus granulosus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q869D7 (Residues: 34-617; Coverage: 94%)
Gene name: TGR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand AU 2 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 109.346Å b: 109.346Å c: 258.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.249
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

6 citation in other articles

Repurposing Auranofin and Evaluation of a New Gold(I) Compound for the Search of Treatment of Human and Cattle Parasitic Diseases: From Protozoa to Helminth Infections.
Feng et al. (2020)
5 more

3 mentions without citation

HEPN RNases - an emerging class of functionally distinct RNA processing and degradation enzymes.
Pillon et al. (2021)
2 more