› 5w37

X-ray diffraction
2.52Å resolution

Crystal structure of mutant CJ YCEI protein (CJ-N182C) for nanotechnology applications

Released:
DOI: 10.2210/pdb5w37/pdb
PDB entry 5w37 coloured by chain, front view.
PDB entry 5w37 coloured by chain, side view.
PDB entry 5w37 coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.
Huber TR, McPherson EC, Keating CE, Snow CD
Bioconjug Chem 29 17-22 (2018)
PMID: 29232505

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-171051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid/polyisoprenoid-binding YceI-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.19 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0PB90 (Residues: 22-190; Coverage: 100%)
Gene name: Cj0420
Sequence domains: YceI-like domain
Structure domains: Lipid/polyisoprenoid-binding, YceI-like

Ligands and Environments

2 bound ligands:
Ligand LFA 1 x LFA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P622
Unit cell:
a: 178.429Å b: 178.429Å c: 50.583Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Protein Assembly by Design.
Zhu et al. (2021)
2 more