› 5w3n

Solid-state NMR

Molecular structure of FUS low sequence complexity domain protein fibrils

Released:
DOI: 10.2210/pdb5w3n/pdb
PDB entry 5w3n coloured by chain, ensemble of 20 models, front view.
PDB entry 5w3n coloured by chain, ensemble of 20 models, side view.
PDB entry 5w3n coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of FUS Protein Fibrils and Its Relevance to Self-Assembly and Phase Separation of Low-Complexity Domains.
Murray DT, Kato M, Lin Y, Thurber KR, Hung I, McKnight SL, Tycko R
Cell 171 615-627.e16 (2017)
PMID: 28942918

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein FUS Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 241 amino acids
Theoretical weight: 24.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P35637 (Residues: 2-214; Coverage: 41%)
Gene names: FUS, TLS

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 4%
Refinement method: simulated annealing
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

122 review citations

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates.
Alberti et al. (2019)
121 more

15 mentions without citation

Biomolecular Condensates in the Nucleus.
Sabari et al. (2020)
14 more