› 5w3s

Electron Microscopy
2.94Å resolution

Cryo-electron microscopy structure of a TRPML3 ion channel

Released:
DOI: 10.2210/pdb5w3s/pdb
PDB entry 5w3s coloured by chain, front view.
PDB entry 5w3s coloured by chain, side view.
PDB entry 5w3s coloured by chain, top view.
Source organism: Callithrix jacchus
Primary publication:
Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3.
Hirschi M, Herzik MA, Wie J, Suo Y, Borschel WF, Ren D, Lander GC, Lee SY
Nature 550 411-414 (2017)
PMID: 29019979
Related structures: EMD-8764

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mucolipin-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 561 amino acids
Theoretical weight: 65.09 KDa
Source organism: Callithrix jacchus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: F6RG56 (Residues: 1-553; Coverage: 100%)
Gene name: MCOLN3
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand Y01 12 x Y01
Ligand NA 6 x NA
Ligand 3PE 4 x 3PE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.94Å
Relevant EMDB volumes: EMD-8764
Expression system: Spodoptera frugiperda

Quick links

Citations

24 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
23 more

16 mentions without citation

The role of π-helices in TRP channel gating.
Zubcevic et al. (2019)
15 more