› 5w44

X-ray diffraction
2.1Å resolution

Crystal structure of the influenza virus PA endonuclease in complex with inhibitor 7a (SRI-29770)

Released:
DOI: 10.2210/pdb5w44/pdb
PDB entry 5w44 coloured by chain, front view.
PDB entry 5w44 coloured by chain, side view.
PDB entry 5w44 coloured by chain, top view.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
Protein-Structure Assisted Optimization of 4,5-Dihydroxypyrimidine-6-Carboxamide Inhibitors of Influenza Virus Endonuclease.
Beylkin D, Kumar G, Zhou W, Park J, Jeevan T, Lagisetti C, Harfoot R, Webby RJ, White SW, Webb TR
Sci Rep 7 17139 (2017)
PMID: 29215062

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 21.05 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: C3W5S0 (Residues: 1-50, 73-196; Coverage: 24%)
Gene name: PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

4 bound ligands:
Ligand MN 1 x MN
Ligand GY6 1 x GY6
Ligand MG 1 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6422
Unit cell:
a: 74.111Å b: 74.111Å c: 127.845Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.224
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Structural insights into the substrate specificity of the endonuclease activity of the influenza virus cap-snatching mechanism.
Kumar et al. (2021)
3 more

3 mentions without citation

New anti-viral drugs for the treatment of COVID-19 instead of favipiravir.
Aktaş et al. (2021)
2 more