› 5w4a

X-ray diffraction
1.5Å resolution

C. japonica N-domain

Released:
DOI: 10.2210/pdb5w4a/pdb
PDB entry 5w4a coloured by chain, front view.
PDB entry 5w4a coloured by chain, side view.
PDB entry 5w4a coloured by chain, top view.
Source organism: Caenorhabditis japonica
Primary publication:
C. elegans germ granules require both assembly and localized regulators for mRNA repression.
Aoki ST, Lynch TR, Crittenden SL, Bingman CA, Wickens M, Kimble J
Nat Commun 12 996 (2021)
PMID: 33579952

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166242 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
P-granule scaffold Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 216 amino acids
Theoretical weight: 24.37 KDa
Source organism: Caenorhabditis japonica
Expression system: Escherichia coli BL21

Ligands and Environments

5 bound ligands:
Ligand EDO 11 x EDO
Ligand PGE 4 x PGE
Ligand IMD 5 x IMD
Ligand PG4 1 x PG4
Ligand PEG 2 x PEG
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 132.77Å b: 94.67Å c: 72.95Å
α: 90° β: 90.76° γ: 90°
R-values:
R R work R free
0.162 0.162 0.197
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

The Dynamic Regulation of mRNA Translation and Ribosome Biogenesis During Germ Cell Development and Reproductive Aging.
Mercer et al. (2021)
6 more

1 mention without citation

C. elegans germ granules require both assembly and localized regulators for mRNA repression.
Aoki et al. (2021)