› 5w5n

X-ray diffraction
1.85Å resolution

Crystal structure of human IgG4-Sigma2 Fc fragment

Released:
DOI: 10.2210/pdb5w5n/pdb
PDB entry 5w5n coloured by chain, front view.
PDB entry 5w5n coloured by chain, side view.
PDB entry 5w5n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional, Biophysical, and Structural Characterization of Human IgG1 and IgG4 Fc Variants with Ablated Immune Functionality.
Tam SH, McCarthy SG, Armstrong AA, Somani S, Wu SJ, Liu X, Gervais A, Ernst R, Saro D, Decker R, Luo J, Gilliland GL, Chiu ML, Scallon BJ
Antibodies (Basel) 6 (2017)
PMID: 31548527

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Immunoglobulin heavy constant gamma 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 24.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01861 (Residues: 106-325; Coverage: 55%)
Gene name: IGHG4
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUC
Carbohydrate polymer
2 bound ligands:
Ligand GOL 2 x GOL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 74.52Å b: 78.47Å c: 97.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.215
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Considerations for the Design of Antibody-Based Therapeutics.
Goulet et al. (2020)
4 more

1 mention without citation

Functional, Biophysical, and Structural Characterization of Human IgG1 and IgG4 Fc Variants with Ablated Immune Functionality.
Tam et al. (2017)