› 5w7d

X-ray diffraction
1.75Å resolution

Murine acyloxyacyl hydrolase (AOAH), S262A mutant

Released:
DOI: 10.2210/pdb5w7d/pdb
PDB entry 5w7d coloured by chain, front view.
PDB entry 5w7d coloured by chain, side view.
PDB entry 5w7d coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the mammalian lipopolysaccharide detoxifier.
Gorelik A, Illes K, Nagar B
Proc Natl Acad Sci U S A 115 E896-E905 (2018)
PMID: 29343645

Function and Biology Details

Reaction catalysed: 
3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128669 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Acyloxyacyl hydrolase Chain: A
Molecule details ›
Chain: A
Length: 562 amino acids
Theoretical weight: 64.03 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O35298 (Residues: 23-574; Coverage: 100%)
Gene name: Aoah
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand CA 3 x CA
Ligand NAG 1 x NAG
Ligand GOL 1 x GOL
Ligand PX8 1 x PX8
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 46.527Å b: 98.59Å c: 73.488Å
α: 90° β: 99.68° γ: 90°
R-values:
R R work R free
0.154 0.152 0.182
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling.
Ciesielska et al. (2021)
1 more

2 mentions without citation

Crystal structure of the mammalian lipopolysaccharide detoxifier.
Gorelik et al. (2018)
1 more