› 5wi9

X-ray diffraction
2.7Å resolution

Crystal structure of KL with an agonist Fab

Released:
DOI: 10.2210/pdb5wi9/pdb
PDB entry 5wi9 coloured by chain, front view.
PDB entry 5wi9 coloured by chain, side view.
PDB entry 5wi9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Agonistic β-Klotho antibody mimics fibroblast growth factor 21 (FGF21) functions.
Min X, Weiszmann J, Johnstone S, Wang W, Yu X, Romanow W, Thibault S, Li Y, Wang Z
J Biol Chem 293 14678-14688 (2018)
PMID: 30068552

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212555 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-klotho Chains: A, B
Molecule details ›
Chains: A, B
Length: 470 amino acids
Theoretical weight: 54.47 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q86Z14 (Residues: 52-508; Coverage: 44%)
Gene name: KLB
Sequence domains: Glycosyl hydrolase family 1
39F7 Fab light chain Chains: E, L
Molecule details ›
Chains: E, L
Length: 215 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
39F7 Fab heavy chain Chains: F, H
Molecule details ›
Chains: F, H
Length: 225 amino acids
Theoretical weight: 24.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

4 bound ligands:
Ligand NAG 4 x NAG
Ligand EDO 7 x EDO
Ligand SO4 2 x SO4
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 118.018Å b: 68.465Å c: 147.811Å
α: 90° β: 111.86° γ: 90°
R-values:
R R work R free
0.218 0.216 0.27
Expression systems:
  • Baculovirus expression vector pFastBac1-HM
  • Homo sapiens

Quick links

Citations

7 review citations

New developments in the biology of fibroblast growth factors.
Ornitz et al. (2022)
6 more

2 mentions without citation

Agonistic β-Klotho antibody mimics fibroblast growth factor 21 (FGF21) functions.
Min et al. (2018)
1 more