› 5wq8

Electron Microscopy
3.26Å resolution

CryoEM structure of type II secretion system secretin GspD in Vibrio cholerae

Released:
DOI: 10.2210/pdb5wq8/pdb
PDB entry 5wq8 coloured by chain, front view.
PDB entry 5wq8 coloured by chain, side view.
PDB entry 5wq8 coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
Structural insights into the secretin translocation channel in the type II secretion system.
Yan Z, Yin M, Xu D, Zhu Y, Li X
Nat Struct Mol Biol 24 177-183 (2017)
PMID: 28067918
Related structures: EMD-6676

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155345 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Secretin GspD Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Length: 650 amino acids
Theoretical weight: 70.86 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P45779 (Residues: 25-674; Coverage: 100%)
Gene names: VC_2733, epsD
Sequence domains:
Structure domains: Ribosomal Protein S8; Chain: A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.26Å
Relevant EMDB volumes: EMD-6676
Expression system: Escherichia coli K-12

Quick links

Citations

14 review citations

A comprehensive guide to pilus biogenesis in Gram-negative bacteria.
Hospenthal et al. (2017)
13 more

9 mentions without citation

Super-Resolution Imaging of Protein Secretion Systems and the Cell Surface of Gram-Negative Bacteria.
Gunasinghe et al. (2017)
8 more