› 5wrs

X-ray diffraction
2.75Å resolution

Crystal Structure of Fam20A in complex with ATP

Released:
DOI: 10.2210/pdb5wrs/pdb
PDB entry 5wrs coloured by chain, front view.
PDB entry 5wrs coloured by chain, side view.
PDB entry 5wrs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Fam20A reveals a pseudokinase featuring a unique disulfide pattern and inverted ATP-binding.
Cui J, Zhu Q, Zhang H, Cianfrocco MA, Leschziner AE, Dixon JE, Xiao J
Elife 6 (2017)
PMID: 28432788

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Pseudokinase FAM20A Chains: A, B
Molecule details ›
Chains: A, B
Length: 438 amino acids
Theoretical weight: 50.43 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96MK3 (Residues: 89-526; Coverage: 86%)
Gene names: FAM20A, UNQ9388/PRO34279
Sequence domains: Golgi casein kinase, C-terminal, Fam20

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 157.202Å b: 157.202Å c: 144.802Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.203 0.238
Expression system: Trichoplusia ni

Quick links

Citations

6 review citations

Live and let die: insights into pseudoenzyme mechanisms from structure.
Murphy et al. (2017)
5 more

2 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
1 more