› 5wrv

X-ray diffraction
1.7Å resolution

Complex structure of human SRP72/SRP68

Released:
DOI: 10.2210/pdb5wrv/pdb
PDB entry 5wrv coloured by chain, front view.
PDB entry 5wrv coloured by chain, side view.
PDB entry 5wrv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human apo-SRP72 and SRP68/72 complex structures reveal the molecular basis of protein translocation.
Gao Y, Zhang Q, Lang Y, Liu Y, Dong X, Chen Z, Tian W, Tang J, Wu W, Tong Y, Chen Z
J Mol Cell Biol 9 220-230 (2017)
PMID: 28369529

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131216 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Signal recognition particle subunit SRP68 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHB9 (Residues: 509-614; Coverage: 17%)
Gene name: SRP68
Signal recognition particle subunit SRP72 Chain: B
Molecule details ›
Chain: B
Length: 163 amino acids
Theoretical weight: 18.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O76094 (Residues: 1-163; Coverage: 24%)
Gene name: SRP72
Sequence domains: Putative TPR-like repeat

Ligands and Environments

4 bound ligands:
Ligand ACT 2 x ACT
Ligand GOL 5 x GOL
Ligand SO4 2 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P6322
Unit cell:
a: 120.598Å b: 120.598Å c: 80.001Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.164 0.189
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Structure of Rift Valley Fever Virus RNA-Dependent RNA Polymerase.
Wang et al. (2022)
8 more

4 mentions without citation

SRPassing Co-translational Targeting: The Role of the Signal Recognition Particle in Protein Targeting and mRNA Protection.
Kellogg et al. (2021)
3 more