› 5wti

X-ray diffraction
2.68Å resolution

Crystal structure of the CRISPR-associated protein in complex with crRNA and DNA

Released:
DOI: 10.2210/pdb5wti/pdb
PDB entry 5wti coloured by chain, front view.
PDB entry 5wti coloured by chain, side view.
PDB entry 5wti coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of stringent PAM recognition by CRISPR-C2c1 in complex with sgRNA.
Wu D, Guan X, Zhu Y, Ren K, Huang Z
Cell Res 27 705-708 (2017)
PMID: 28374750

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-242527 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated protein Chain: Z
Molecule details ›
Chain: Z
Length: 1108 amino acids
Theoretical weight: 130.57 KDa
Source organism: Caldibacillus thermoamylovorans
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0D0F5I0 (Residues: 1-1108; Coverage: 100%)
DNA (28-MER) Chain: E
Molecule details ›
Chain: E
Length: 29 nucleotides
Theoretical weight: 8.78 KDa
Source organism: Lobariella soredians
Expression system: Not provided
DNA (5'-D(P*GP*TP*GP*TP*GP*GP*AP*TP*TP*CP*CP*G)-3') Chain: H
Molecule details ›
Chain: H
Length: 12 nucleotides
Theoretical weight: 3.71 KDa
Source organism: Lobariella soredians
Expression system: Not provided
RNA (123-MER) Chain: B
Molecule details ›
Chain: B
Length: 123 nucleotides
Theoretical weight: 39.66 KDa
Source organism: RNA transcription vector pBRDI1
Expression system: RNA transcription vector pBRDI1

Ligands and Environments

1 bound ligand:
Ligand MG 26 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 172.056Å b: 138.742Å c: 95.611Å
α: 90° β: 117.45° γ: 90°
R-values:
R R work R free
0.218 0.217 0.264
Expression systems:
  • Escherichia coli
  • Not provided
  • RNA transcription vector pBRDI1

Quick links

Citations

6 review citations

The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit.
Murugan et al. (2017)
5 more

5 mentions without citation

Structural basis of stringent PAM recognition by CRISPR-C2c1 in complex with sgRNA.
Wu et al. (2017)
4 more