› 5wxh

X-ray diffraction
1.3Å resolution

Crystal structure of TAF3 PHD finger bound to H3K4me3

Released:
DOI: 10.2210/pdb5wxh/pdb
PDB entry 5wxh coloured by chain, front view.
PDB entry 5wxh coloured by chain, side view.
PDB entry 5wxh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology.
Zhao S, Yang M, Zhou W, Zhang B, Cheng Z, Huang J, Zhang M, Wang Z, Wang R, Chen Z, Zhu J, Li H
Proc Natl Acad Sci U S A 114 E7245-E7254 (2017)
PMID: 28808021

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159415 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription initiation factor TFIID subunit 3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5VWG9 (Residues: 853-915; Coverage: 7%)
Gene name: TAF3
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1 Chains: D, P
Molecule details ›
Chains: D, P
Length: 7 amino acids
Theoretical weight: 819 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-8; Coverage: 5%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
1 modified residue:
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 27.244Å b: 35.138Å c: 38.562Å
α: 82.96° β: 75.62° γ: 73.49°
R-values:
R R work R free
0.154 0.153 0.175
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

DNA mismatch repair in the context of chromatin.
Huang et al. (2020)
6 more

4 mentions without citation

Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology.
Zhao et al. (2017)
3 more