› 5x3t

X-ray diffraction
2.65Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129469 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Antitoxin VapB26 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 71 amino acids
Theoretical weight: 7.73 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: O53778 (Residues: 1-71; Coverage: 100%)
Gene names: Rv0581, vapB26
Sequence domains: Ribbon-helix-helix protein, copG family
Ribonuclease VapC26 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 155 amino acids
Theoretical weight: 16.78 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: O53779 (Residues: 1-135; Coverage: 100%)
Gene names: Rv0582, vapC26
Sequence domains: PIN domain

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P41
Unit cell:
a: 64.221Å b: 64.221Å c: 216.134Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.239
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Regulation of Three Virulence Strategies of Mycobacterium tuberculosis: A Success Story.
Zondervan et al. (2018)
2 more

6 mentions without citation

A Systematic Overview of Type II and III Toxin-Antitoxin Systems with a Focus on Druggability.
Kang et al. (2018)
5 more