› 5x3z

Solution NMR

Solution structure of musashi1 RBD2 in complex with RNA

Released:
DOI: 10.2210/pdb5x3z/pdb
PDB entry 5x3z coloured by chain, ensemble of 20 models, front view.
PDB entry 5x3z coloured by chain, ensemble of 20 models, side view.
PDB entry 5x3z coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Primary publication:
Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA.
Iwaoka R, Nagata T, Tsuda K, Imai T, Okano H, Kobayashi N, Katahira M
Molecules 22 (2017)
PMID: 28753936

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178889 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein Musashi homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 10.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q61474 (Residues: 109-200; Coverage: 25%)
Gene names: Msi1, Msi1h
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*GP*UP*AP*GP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.59 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 83%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

RNA-Targeted Therapies and High-Throughput Screening Methods.
Zhu et al. (2020)
2 more

5 mentions without citation

Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA.
Iwaoka et al. (2017)
4 more