› 5x4b

X-ray diffraction
1.5Å resolution

Crystal Structure of N-terminal G-domain of EngA from Bacillus subtilis

Released:
DOI: 10.2210/pdb5x4b/pdb
PDB entry 5x4b coloured by chain, front view.
PDB entry 5x4b coloured by chain, side view.
PDB entry 5x4b coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Entry authors: Singh V, Prakash B

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase Der Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 17.78 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P50743 (Residues: 2-163; Coverage: 37%)
Gene names: BSU22840, der, engA, yphC
Sequence domains: 50S ribosome-binding GTPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GDP 2 x GDP
Ligand K 3 x K
Ligand MG 2 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 42.26Å b: 43.75Å c: 50.71Å
α: 66.88° β: 79.88° γ: 70.82°
R-values:
R R work R free
0.154 0.153 0.181
Expression system: Escherichia coli

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