› 5x5i

X-ray diffraction
2.55Å resolution

The X-ray crystal structure of a TetR family transcription regulator RcdA involved in the regulation of biofilm formation in Escherichia coli

Released:
DOI: 10.2210/pdb5x5i/pdb
PDB entry 5x5i coloured by chain, front view.
PDB entry 5x5i coloured by chain, side view.
PDB entry 5x5i coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A structural sketch of RcdA, a transcription factor controlling the master regulator of biofilm formation.
Sugino H, Usui T, Shimada T, Nakano M, Ogasawara H, Ishihama A, Hirata A
FEBS Lett 591 2019-2031 (2017)
PMID: 28608551

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160001 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator RcdA Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 189 amino acids
Theoretical weight: 22.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P75811 (Residues: 1-178; Coverage: 100%)
Gene names: JW5114, b0846, rcdA, ybjK
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 80 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P1
Unit cell:
a: 38.477Å b: 75.967Å c: 110.051Å
α: 90° β: 90.01° γ: 89.95°
R-values:
R R work R free
0.201 0.198 0.266
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Regulatory role of CsqR (YihW) in transcription of the genes for catabolism of the anionic sugar sulfoquinovose (SQ) in Escherichia coli K-12.
Shimada et al. (2019)
2 more