› 5x6s

X-ray diffraction
1.9Å resolution

Acetyl xylan esterase from Aspergillus awamori

Released:
DOI: 10.2210/pdb5x6s/pdb
PDB entry 5x6s coloured by chain, front view.
PDB entry 5x6s coloured by chain, side view.
PDB entry 5x6s coloured by chain, top view.
Source organism: Aspergillus awamori
Primary publication:
Crystal Structure and Substrate Specificity Modification of Acetyl Xylan Esterase from Aspergillus luchuensis.
Komiya D, Hori A, Ishida T, Igarashi K, Samejima M, Koseki T, Fushinobu S
Appl Environ Microbiol 83 (2017)
PMID: 28802264

Function and Biology Details

Reaction catalysed: 
Deacetylation of xylans and xylo-oligosaccharides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187640 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Acetylxylan esterase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 29.53 KDa
Source organism: Aspergillus awamori
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q92194 (Residues: 30-304; Coverage: 98%)
Gene names: aceA, axeA
Sequence domains: Esterase PHB depolymerase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand MES 3 x MES
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: R3
Unit cell:
a: 174.151Å b: 174.151Å c: 50.708Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.151 0.185
Expression system: Komagataella pastoris

Quick links

Citations

8 citation in other articles

A De Novo Designed Esterase with p-Nitrophenyl Acetate Hydrolysis Activity.
Li et al. (2020)
7 more

2 mentions without citation

Functional Validation of Two Fungal Subfamilies in Carbohydrate Esterase Family 1 by Biochemical Characterization of Esterases From Uncharacterized Branches.
Li et al. (2020)
1 more