› 5x88

X-ray diffraction
1.76Å resolution

A crystal structure of cutinases from Malbranchea cinnamomea

Released:
DOI: 10.2210/pdb5x88/pdb
PDB entry 5x88 coloured by chain, front view.
PDB entry 5x88 coloured by chain, side view.
PDB entry 5x88 coloured by chain, top view.
Source organism: Malbranchea cinnamomea
Primary publication:
High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production.
Duan X, Liu Y, You X, Jiang Z, Yang S, Yang S
Biotechnol Biofuels 10 223 (2017)
PMID: 28932264

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103736 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 21.2 KDa
Source organism: Malbranchea cinnamomea
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: A0A1S6YJF3 (Residues: 17-215; Coverage: 100%)
Sequence domains: Cutinase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Unit cell:
a: 34.407Å b: 37.815Å c: 127.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.175
Expression system: Komagataella phaffii GS115

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Citations

3 review citations

Bioprospecting of gut microflora for plastic biodegradation.
Cf et al. (2021)
2 more