› 5xbz

X-ray diffraction
2.7Å resolution

Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose

Released:
DOI: 10.2210/pdb5xbz/pdb
PDB entry 5xbz coloured by chain, front view.
PDB entry 5xbz coloured by chain, side view.
PDB entry 5xbz coloured by chain, top view.
Source organism: Rhizomucor miehei
Entry authors: Yang S, Qin Z, Zhou P, Yan Q, Jiang Z

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Glucan endo-1,3-beta-D-glucosidase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 796 amino acids
Theoretical weight: 89.59 KDa
Source organism: Rhizomucor miehei
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A023I7E1 (Residues: 1-796; Coverage: 100%)
Gene names: ENG1, LAM81A
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P21
Unit cell:
a: 52.916Å b: 96.958Å c: 127.642Å
α: 90° β: 93.91° γ: 90°
R-values:
R R work R free
0.178 0.174 0.19
Expression system: Escherichia coli

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