› 5xd0

X-ray diffraction
1.79Å resolution

Apo Structure of Beta-1,3-1,4-glucanase from Paenibacillus sp.X4

Released:
DOI: 10.2210/pdb5xd0/pdb
PDB entry 5xd0 coloured by chain, front view.
PDB entry 5xd0 coloured by chain, side view.
PDB entry 5xd0 coloured by chain, top view.
Source organism: Paenibacillus sp. X4
Primary publication:
Improvement of enzyme activity of β-1,3-1,4-glucanase from Paenibacillus sp. X4 by error-prone PCR and structural insights of mutated residues.
Baek SC, Ho TH, Lee HW, Jung WK, Gang HS, Kang LW, Kim H
Appl Microbiol Biotechnol 101 4073-4083 (2017)
PMID: 28180917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 44.98 KDa
Source organism: Paenibacillus sp. X4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A088BCU2 (Residues: 1-409; Coverage: 100%)
Gene name: lic8H
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

2 bound ligands:
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P1
Unit cell:
a: 46.721Å b: 64.161Å c: 70.502Å
α: 71.04° β: 77.07° γ: 75.6°
R-values:
R R work R free
0.153 0.151 0.188
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
Huang et al. (2021)
2 more

4 mentions without citation

Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
Romero Victorica et al. (2020)
3 more