› 5xhb

X-ray diffraction
1.9Å resolution

Crystal structure of the full length of NisI in a lipid free form, the nisin immunity protein, from Lactococcus lactis

Released:
DOI: 10.2210/pdb5xhb/pdb
PDB entry 5xhb coloured by chain, front view.
PDB entry 5xhb coloured by chain, side view.
PDB entry 5xhb coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Primary publication:
Crystal Structure of NisI in a Lipid-Free Form, the Nisin Immunity Protein, from Lactococcus lactis.
Jeong JH, Ha SC
Antimicrob Agents Chemother 62 (2018)
PMID: 29311076

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nisin immunity protein Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 26.32 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42708 (Residues: 22-245; Coverage: 99%)
Gene name: nisI
Sequence domains: Lantibiotic immunity protein Spa1 C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 45.984Å b: 76.866Å c: 76.667Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Lipoproteins in Gram-Positive Bacteria: Abundance, Function, Fitness.
Nguyen et al. (2020)
3 more

4 mentions without citation

Probiotics-Derived Peptides and Their Immunomodulatory Molecules Can Play a Preventive Role Against Viral Diseases Including COVID-19.
Manna et al. (2021)
3 more