› 5xk2

X-ray diffraction
1.7Å resolution

Crystal structure of mono- and diacylglycerol lipase from Aspergillus oryzae

Released:
DOI: 10.2210/pdb5xk2/pdb
PDB entry 5xk2 coloured by chain, front view.
PDB entry 5xk2 coloured by chain, side view.
PDB entry 5xk2 coloured by chain, top view.
Source organism: Aspergillus oryzae
Entry authors: Wang YH, Lan DM

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
feruloyl esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 31.39 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: P78583 (Residues: 21-306; Coverage: 100%)
Gene names: OAory_01019780, mdlB
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 47.329Å b: 67.277Å c: 89.574Å
α: 90° β: 95.21° γ: 90°
R-values:
R R work R free
0.142 0.141 0.168
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

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