› 5xmi

Electron Microscopy
3.9Å resolution

Cryo-EM Structure of the ATP-bound VPS4 mutant-E233Q hexamer (masked)

Released:
DOI: 10.2210/pdb5xmi/pdb
PDB entry 5xmi coloured by chain, front view.
PDB entry 5xmi coloured by chain, side view.
PDB entry 5xmi coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution.
Sun S, Li L, Yang F, Wang X, Fan F, Yang M, Chen C, Li X, Wang HW, Sui SF
Nat Commun 8 16064 (2017)
PMID: 28714467
Related structures: EMD-6733

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 4 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 437 amino acids
Theoretical weight: 48.23 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P52917 (Residues: 1-437; Coverage: 100%)
Gene names: CSC1, DID6, END13, GRD13, P9705.10, VPL4, VPS4, VPT10, YPR173C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 5 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-6733
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structures, Functions, and Dynamics of ESCRT-III/Vps4 Membrane Remodeling and Fission Complexes.
McCullough et al. (2018)
8 more

2 mentions without citation

Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution.
Sun et al. (2017)
1 more