› 5xmw

X-ray diffraction
3Å resolution

Selenomethionine-derivated ZHD

Released:
DOI: 10.2210/pdb5xmw/pdb
PDB entry 5xmw coloured by chain, front view.
PDB entry 5xmw coloured by chain, side view.
PDB entry 5xmw coloured by chain, top view.
Source organism: Clonostachys rosea
Primary publication:
The structure of a complex of the lactonohydrolase zearalenone hydrolase with the hydrolysis product of zearalenone at 1.60 Å resolution.
Qi Q, Yang WJ, Zhou HJ, Ming DM, Sun KL, Xu TY, Hu XJ, Lv H
Acta Crystallogr F Struct Biol Commun 73 376-381 (2017)
PMID: 28695844

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 31.35 KDa
Source organism: Clonostachys rosea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NKB0 (Residues: 1-264; Coverage: 100%)
Gene name: zhd101
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 73.924Å b: 90.169Å c: 113.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.236
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Detoxification Strategies for Zearalenone Using Microorganisms: A Review.
Wang et al. (2019)
2 more

1 mention without citation

The structure of a complex of the lactonohydrolase zearalenone hydrolase with the hydrolysis product of zearalenone at 1.60 Å resolution.
Qi et al. (2017)