› 5xo7

X-ray diffraction
1.88Å resolution

Crystal structure of a novel ZEN lactonase mutant with ligand a

Released:
DOI: 10.2210/pdb5xo7/pdb
PDB entry 5xo7 coloured by chain, front view.
PDB entry 5xo7 coloured by chain, side view.
PDB entry 5xo7 coloured by chain, top view.
Source organism: Rhinocladiella mackenziei CBS 650.93
Primary publication:
Crystal Structure of a Mycoestrogen-Detoxifying Lactonase from Rhinocladiella mackenziei: Molecular Insight into ZHD Substrate Selectivity
Zheng YY, Liu WT, Chen CC, Hu XY, Liu WD, Ko TP, Tang XK, Wei HL, Huang JW, Guo RT
ACS Catalysis 8 4294-4298 (2018)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 266 amino acids
Theoretical weight: 29.45 KDa
Source organism: Rhinocladiella mackenziei CBS 650.93
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0D2ILK1 (Residues: 1-266; Coverage: 100%)
Gene name: Z518_04590
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
Ligand 36J 8 x 36J
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P1
Unit cell:
a: 75.462Å b: 95.158Å c: 101.444Å
α: 90.2° β: 92.13° γ: 91.6°
R-values:
R R work R free
0.156 0.156 0.195
Expression system: Escherichia coli BL21(DE3)

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