5xrz

X-ray diffraction
3.6Å resolution

Structure of a ssDNA bound to the inner DNA binding site of RAD52

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-119406 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA repair protein RAD52 homolog Chains: A, B, C, D, E, F, G, H, I, J, K
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K
Length: 215 amino acids
Theoretical weight: 23.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43351 (Residues: 1-212; Coverage: 51%)
Gene name: RAD52
Sequence domains: Rad52/22 family double-strand break repair protein
Structure domains: DNA repair protein Rad52/59/22
ssDNA (40-MER) Chain: L
Molecule details ›
Chain: L
Length: 40 nucleotides
Theoretical weight: 12.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P1
Unit cell:
a: 67.621Å b: 101.42Å c: 101.71Å
α: 91.89° β: 108.68° γ: 108.17°
R-values:
R R work R free
0.219 0.216 0.256
Expression systems:
  • Escherichia coli
  • Not provided