› 5xvo

X-ray diffraction
3.1Å resolution

E. fae Cas1-Cas2/prespacer/target ternary complex revealing DNA sampling and half-integration states

Released:
DOI: 10.2210/pdb5xvo/pdb
PDB entry 5xvo coloured by chain, front view.
PDB entry 5xvo coloured by chain, side view.
PDB entry 5xvo coloured by chain, top view.
Source organism: Enterococcus faecalis TX0027
Primary publication:
How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration.
Xiao Y, Ng S, Nam KH, Ke A
Nature 550 137-141 (2017)
PMID: 28869593

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero decamer (preferred)
hetero octamer
hetero octadecamer
PDBe Complex ID:
PDB-CPX-119411 (preferred)
Entry contents:
2 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (6 distinct):
CRISPR-associated endonuclease Cas1 Chains: A, B, C, D, I, J, K, L
Molecule details ›
Chains: A, B, C, D, I, J, K, L
Length: 288 amino acids
Theoretical weight: 33.49 KDa
Source organism: Enterococcus faecalis TX0027
Expression system: Escherichia coli
CRISPR-associated endoribonuclease Cas2 Chains: E, F, M, N
Molecule details ›
Chains: E, F, M, N
Length: 109 amino acids
Theoretical weight: 12.98 KDa
Source organism: Enterococcus faecalis TX0027
Expression system: Escherichia coli
DNA (28-MER) Chains: G, O, P
Molecule details ›
Chains: G, O, P
Length: 28 nucleotides
Theoretical weight: 8.55 KDa
DNA (5'-D(P*CP*CP*GP*AP*G)-3') Chain: H
Molecule details ›
Chain: H
Length: 5 nucleotides
Theoretical weight: 1.51 KDa
DNA (46-MER) Chain: Q
Molecule details ›
Chain: Q
Length: 46 nucleotides
Theoretical weight: 14.09 KDa
DNA (69-MER) Chain: R
Molecule details ›
Chain: R
Length: 69 nucleotides
Theoretical weight: 21.28 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 131.893Å b: 124.8Å c: 157.905Å
α: 90° β: 106.5° γ: 90°
R-values:
R R work R free
0.196 0.194 0.233
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Molecular mechanisms of CRISPR-Cas spacer acquisition.
McGinn et al. (2019)
11 more

5 mentions without citation

Creating memories: molecular mechanisms of CRISPR adaptation.
Lee et al. (2022)
4 more