› 5xvp

X-ray diffraction
3Å resolution

E. fae Cas1-Cas2/prespacer/target ternary complex revealing the fully integrated states

Released:
DOI: 10.2210/pdb5xvp/pdb
PDB entry 5xvp coloured by chain, front view.
PDB entry 5xvp coloured by chain, side view.
PDB entry 5xvp coloured by chain, top view.
Source organism: Enterococcus faecalis TX0027
Primary publication:
How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration.
Xiao Y, Ng S, Nam KH, Ke A
Nature 550 137-141 (2017)
PMID: 28869593

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-119414 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 288 amino acids
Theoretical weight: 33.49 KDa
Source organism: Enterococcus faecalis TX0027
Expression system: Escherichia coli
CRISPR-associated endoribonuclease Cas2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 109 amino acids
Theoretical weight: 12.98 KDa
Source organism: Enterococcus faecalis TX0027
Expression system: Escherichia coli
DNA (73-MER) Chain: G
Molecule details ›
Chain: G
Length: 73 nucleotides
Theoretical weight: 22.41 KDa
DNA (73-MER) Chain: H
Molecule details ›
Chain: H
Length: 73 nucleotides
Theoretical weight: 22.55 KDa
DNA (5'-D(P*TP*TP*CP*TP*CP*CP*GP*AP*G)-3') Chains: I, J
Molecule details ›
Chains: I, J
Length: 9 nucleotides
Theoretical weight: 2.71 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 64.87Å b: 212.294Å c: 512.199Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.233
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Molecular mechanisms of CRISPR-Cas spacer acquisition.
McGinn et al. (2019)
11 more

9 mentions without citation

Creating memories: molecular mechanisms of CRISPR adaptation.
Lee et al. (2022)
8 more