› 5xwy

Electron Microscopy
3.2Å resolution

Electron cryo-microscopy structure of LbuCas13a-crRNA binary complex

Released:
DOI: 10.2210/pdb5xwy/pdb
PDB entry 5xwy coloured by chain, front view.
PDB entry 5xwy coloured by chain, side view.
PDB entry 5xwy coloured by chain, top view.
Source organisms:
Primary publication:
The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a.
Liu L, Li X, Ma J, Li Z, You L, Wang J, Wang M, Zhang X, Wang Y
Cell 170 714-726.e10 (2017)
PMID: 28757251
Related structures: EMD-6777

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111733 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cas13a Chain: A
Molecule details ›
Chain: A
Length: 1159 amino acids
Theoretical weight: 138.56 KDa
Source organism: Leptotrichia buccalis C-1013-b
Expression system: Escherichia coli
UniProt:
  • Canonical: C7NBY4 (Residues: 1-1159; Coverage: 100%)
Gene names: Lebu_1799, c2c2, cas13a
RNA (59-MER) Chain: B
Molecule details ›
Chain: B
Length: 59 nucleotides
Theoretical weight: 18.87 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-6777
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

62 review citations

The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit.
Murugan et al. (2017)
61 more

9 mentions without citation

Functional Features and Current Applications of the RNA-Targeting Type VI CRISPR-Cas Systems.
Perčulija et al. (2021)
8 more