5xxp

X-ray diffraction
2.55Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-119415 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH lysR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.32 KDa
Source organism: Cupriavidus necator
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WXC7 (Residues: 1-87; Coverage: 30%)
Gene names: BJN34_37375, cbnR
Sequence domains: Bacterial regulatory helix-turn-helix protein, lysR family
DNA (25-MER) Chain: E
Molecule details ›
Chain: E
Length: 25 nucleotides
Theoretical weight: 7.65 KDa
Source organism: Cupriavidus necator
Expression system: Not provided
DNA (25-MER) Chain: F
Molecule details ›
Chain: F
Length: 25 nucleotides
Theoretical weight: 7.7 KDa
Source organism: Cupriavidus necator
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 186.457Å b: 28.719Å c: 73.153Å
α: 90° β: 111.65° γ: 90°
R-values:
R R work R free
0.222 0.219 0.263
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided