› 5xyf

X-ray diffraction
2.2Å resolution

Crystal structure of the human TIN2-TPP1-TRF2 telomeric complex

Released:
DOI: 10.2210/pdb5xyf/pdb
PDB entry 5xyf coloured by chain, front view.
PDB entry 5xyf coloured by chain, side view.
PDB entry 5xyf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analyses of the mammalian TIN2-TPP1-TRF2 telomeric complex.
Hu C, Rai R, Huang C, Broton C, Long J, Xu Y, Xue J, Lei M, Chang S, Chen Y
Cell Res 27 1485-1502 (2017)
PMID: 29160297

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-172316 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
TERF1-interacting nuclear factor 2 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BSI4 (Residues: 2-202; Coverage: 45%)
Gene names: TIN2, TINF2
Sequence domains: TERF1-interacting nuclear factor 2 N-terminus
Telomeric repeat-binding factor 2 Chain: C
Molecule details ›
Chain: C
Length: 18 amino acids
Theoretical weight: 2.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15554 (Residues: 392-408; Coverage: 3%)
Gene names: TERF2, TRBF2, TRF2
Adrenocortical dysplasia protein homolog Chain: B
Molecule details ›
Chain: B
Length: 40 amino acids
Theoretical weight: 4.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96AP0 (Residues: 424-458; Coverage: 8%)
Gene names: ACD, PIP1, PTOP, TINT1, TPP1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2221
Unit cell:
a: 46.222Å b: 112.258Å c: 121.209Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Shelterin-Mediated Telomere Protection.
de Lange T. (2018)
14 more

8 mentions without citation

Structural biology of telomeres and telomerase.
Smith et al. (2020)
7 more