› 5y85

X-ray diffraction
2Å resolution

Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme

Released:
DOI: 10.2210/pdb5y85/pdb
PDB entry 5y85 coloured by chain, front view.
PDB entry 5y85 coloured by chain, side view.
PDB entry 5y85 coloured by chain, top view.
Source organism: metagenome
Primary publication:
Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme.
Zheng L, Mairhofer E, Teplova M, Zhang Y, Ma J, Patel DJ, Micura R, Ren A
Nat Commun 8 1180 (2017)
PMID: 29081514

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113203 (preferred)
Entry contents:
1 distinct RNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
RNA (5'-R(P*AP*CP*CP*CP*GP*CP*AP*AP*GP*GP*CP*CP*GP*AP*CP*GP*GP*C)-3') Chains: A, C
Molecule details ›
Chains: A, C
Length: 18 nucleotides
Theoretical weight: 5.78 KDa
Source organism: metagenome
Expression system: Not provided
DNA/RNA (50-MER) Chains: B, D
Molecule details ›
Chains: B, D
Length: 50 nucleotides
Theoretical weight: 16.09 KDa
Source organism: metagenome
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 26 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 91.182Å b: 109.304Å c: 41.739Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.237
Expression system: Not provided

Quick links

Citations

2 review citations

Classification of the nucleolytic ribozymes based upon catalytic mechanism.
Lilley DMJ. (2019)
1 more

8 mentions without citation

Structure-based mechanistic insights into catalysis by small self-cleaving ribozymes.
Ren et al. (2017)
7 more