› 5yae

X-ray diffraction
2.4Å resolution

Ferulic acid esterase from Streptomyces cinnamoneus at 2.4 A resolution

Released:
DOI: 10.2210/pdb5yae/pdb
PDB entry 5yae coloured by chain, front view.
PDB entry 5yae coloured by chain, side view.
PDB entry 5yae coloured by chain, top view.
Source organism: Streptomyces cinnamoneus
Primary publication:
Loop of Streptomyces Feruloyl Esterase Plays an Important Role in the Enzyme's Catalyzing the Release of Ferulic Acid from Biomass.
Uraji M, Tamura H, Mizohata E, Arima J, Wan K, Ogawa K, Inoue T, Hatanaka T
Appl Environ Microbiol 84 (2018)
PMID: 29150515

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102523 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 37.07 KDa
Source organism: Streptomyces cinnamoneus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M4UW33 (Residues: 42-383; Coverage: 97%)
Gene name: estA
Sequence domains: Platelet-activating factor acetylhydrolase, isoform II

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 53.27Å b: 71.29Å c: 87.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.22
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The release and catabolism of ferulic acid in plant cell wall by rumen microbes: A review.
Wang et al. (2022)
3 other articles

3 mentions without citation

Characterization of Feruloyl Esterase from Bacillus pumilus SK52.001 and Its Application in Ferulic Acid Production from De-Starched Wheat Bran.
Duan et al. (2021)
2 more