› 5ybv

X-ray diffraction
2.12Å resolution

The structure of the KANK2 ankyrin domain with the KIF21A peptide

Released:
DOI: 10.2210/pdb5ybv/pdb
PDB entry 5ybv coloured by chain, front view.
PDB entry 5ybv coloured by chain, side view.
PDB entry 5ybv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the recognition of kinesin family member 21A (KIF21A) by the ankyrin domains of KANK1 and KANK2 proteins.
Guo Q, Liao S, Zhu Z, Li Y, Li F, Xu C
J Biol Chem 293 557-566 (2018)
PMID: 29183992

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179107 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
KN motif and ankyrin repeat domain-containing protein 2 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: Q63ZY3 (Residues: 578-832; Coverage: 30%)
Gene names: ANKRD25, KANK2, KIAA1518, MXRA3, SIP
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain
KN motif and ankyrin repeat domain-containing protein 2 Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 28.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: Q63ZY3 (Residues: 578-832; Coverage: 30%)
Gene names: ANKRD25, KANK2, KIAA1518, MXRA3, SIP
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain
Kinesin-like protein KIF21A Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 amino acids
Theoretical weight: 2.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q7Z4S6 (Residues: 1146-1167; Coverage: 1%)
Gene names: KIAA1708, KIF2, KIF21A

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P1
Unit cell:
a: 45.956Å b: 52.834Å c: 53.478Å
α: 73.02° β: 89.67° γ: 84.91°
R-values:
R R work R free
0.192 0.188 0.235
Expression systems:
  • Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
  • Not provided

Quick links

Citations

2 review citations

Kinase regulation by liquid-liquid phase separation.
López-Palacios et al. (2023)
1 more

1 mention without citation

Structural basis for the recognition of kinesin family member 21A (KIF21A) by the ankyrin domains of KANK1 and KANK2 proteins.
Guo et al. (2018)