5yh3

X-ray diffraction
3.3Å resolution

The structure of hFam20C and hFam20A complex

Released:
Source organism: Homo sapiens
Primary publication:
Structure and evolution of the Fam20 kinases.
Nat Commun 9 1218 (2018)
PMID: 29572475

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-184918 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pseudokinase FAM20A Chains: A, B
Molecule details ›
Chains: A, B
Length: 467 amino acids
Theoretical weight: 53.46 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96MK3 (Residues: 63-529; Coverage: 92%)
Gene names: FAM20A, UNQ9388/PRO34279
Sequence domains: Golgi casein kinase, C-terminal, Fam20
Extracellular serine/threonine protein kinase FAM20C Chains: C, D
Molecule details ›
Chains: C, D
Length: 438 amino acids
Theoretical weight: 50.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8IXL6 (Residues: 141-578; Coverage: 75%)
Gene names: DMP4, FAM20C
Sequence domains: Golgi casein kinase, C-terminal, Fam20

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 78.469Å b: 192.747Å c: 226.669Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.233 0.266
Expression system: Trichoplusia ni