› 5yj2

X-ray diffraction
1.71Å resolution

Crystal structure of Bacillus sp. TB-90 urate oxidase without dehydration

Released:
DOI: 10.2210/pdb5yj2/pdb
PDB entry 5yj2 coloured by chain, front view.
PDB entry 5yj2 coloured by chain, side view.
PDB entry 5yj2 coloured by chain, top view.
Source organism: Bacillus sp. TB-90
Entry authors: Hibi T, Itoh T, Nishiya Y

Function and Biology Details

Reactions catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uric acid degradation bifunctional protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 312 amino acids
Theoretical weight: 35.71 KDa
Source organism: Bacillus sp. TB-90
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q45697 (Residues: 178-489; Coverage: 62%)
Gene name: uao
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

4 bound ligands:
Ligand AZA 4 x AZA
Ligand OXY 4 x OXY
Ligand MXE 3 x MXE
Ligand SO4 2 x SO4
1 modified residue:
Ligand OCS 4 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21212
Unit cell:
a: 133.073Å b: 143.895Å c: 70.231Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.19
Expression system: Escherichia coli DH5[alpha]

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